Search results for "Assembly algorithms"

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Whole genome sequencing data and de novo draft assemblies for 66 teleost species

2017

Teleost fishes comprise more than half of all vertebrate species, yet genomic data are only available for 0.2% of their diversity. Here, we present whole genome sequencing data for 66 new species of teleosts, vastly expanding the availability of genomic data for this important vertebrate group. We report on de novo assemblies based on low-coverage (9–39×) sequencing and present detailed methodology for all analyses. To facilitate further utilization of this data set, we present statistical analyses of the gene space completeness and verify the expected phylogenetic position of the sequenced genomes in a large mitogenomic context. We further present a nuclear marker set used for phylogenetic…

0106 biological sciences0301 basic medicineStatistics and ProbabilityData DescriptorComputational biologyLibrary and Information Sciences010603 evolutionary biology01 natural sciencesGenomeEducation03 medical and health sciencesbiology.animalGenome assembly algorithmsAnimalsDNA sequencingGenePhylogenyGeneticsWhole genome sequencingGenomeWhole Genome SequencingbiologyPhylogenetic treeComparative genomicsGene treeFishesRobustness (evolution)VertebrateGenomicsComputer Science ApplicationsMetadata030104 developmental biologyStatistics Probability and UncertaintyInformation SystemsScientific Data
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An improved genome assembly uncovers prolific tandem repeats in Atlantic cod

2016

AbstractBackground: The first Atlantic cod (Gadus morhua) genome assembly published in 2011 was one of the early genome assemblies exclusively based on high-throughput 454 pyrosequencing. Since then, rapid advances in sequencing technologies have led to a multitude of assemblies generated for complex genomes, although many of these are of a fragmented nature with a significant fraction of bases in gaps. The development of long-read sequencing and improved software now enable the generation of more contiguous genome assemblies.Results: By combining data from Illumina, 454 and the longer PacBio sequencing technologies, as well as integrating the results of multiple assembly programs, we have …

0301 basic medicineHeterozygoteAssembly algorithmsSequence assemblyGenomicsRepetitive DNABiologyGenome03 medical and health sciences0302 clinical medicineAssembly consolidationTandem repeatIndel polymorphismGeneticsAnimalsGadusLong-read sequencing technologyPromoter Regions GeneticMicrosatellitesRepeated sequenceGenePacBioGeneticsHeterozygosityDinucleotide repeatsMolecular Sequence AnnotationGenomicsSequence Analysis DNAbiology.organism_classification030104 developmental biologyGadus morhuaTandem Repeat SequencesEvolutionary biologyPyrosequencingAtlantic cod030217 neurology & neurosurgeryResearch ArticleBiotechnology
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